paav ef1adouble (Addgene inc)
96
Structured Review
Addgene inc
paav ef1adouble
Paav Ef1adouble, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paav+ef1adouble/pAAV-EF1a-double+floxed-hChR2(H134R)-EYFP-WPRE-HGHpA+(Plasmid+%2320298)/pm41077338-58-27-34
Average 96 stars, based on 545 article reviews
Paav Ef1adouble, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paav+ef1adouble/pAAV-EF1a-double+floxed-hChR2(H134R)-EYFP-WPRE-HGHpA+(Plasmid+%2320298)/pm41077338-58-27-34
Average 96 stars, based on 545 article reviews
paav ef1adouble - by Bioz Stars,
2026-10
96/100 stars
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Injection:Article Title: Leptin signaling in the dorsomedial hypothalamus couples breathing and metabolism in obesity. Article Snippet: DREADD (AAV8-hSyn-DIO-hM3D(Gq)-mCherry, Addgene, # 44361-AAV8, 43 1012 vg.mL 1) or Control (AAV8-hSyn-DIO-mCherry, Addgene, #50459-AAV8, 131013 vg.mL 1) virus was delivered bilaterally using pre-pulled glass micropipettes (Sutter, Novato, California) to the DMH using the following stereotactic coordinates from the animal’s bregma: 1.88 mm caudal, ±0.40 mm lateral, and 5.00 mm ventral. .. For optogenetic electrophysiology experiments, Article Title: [1902.06250] Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn Article Snippet: .. Production and intra-spinal injection of rAAV vectors The coding sequence for GCaMP3.NES, targeted to the cytoplasm using a nuclear export signal (NES), was subcloned using standard procedures between the NheI and AscI restriction sites of the Cre recombinase-dependent AAV expression plasmid, Sequencing:Article Title: [1902.06250] Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn Article Snippet: .. Production and intra-spinal injection of rAAV vectors The coding sequence for GCaMP3.NES, targeted to the cytoplasm using a nuclear export signal (NES), was subcloned using standard procedures between the NheI and AscI restriction sites of the Cre recombinase-dependent AAV expression plasmid, Bioprocessing:Article Title: [1902.06250] Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn Article Snippet: .. Production and intra-spinal injection of rAAV vectors The coding sequence for GCaMP3.NES, targeted to the cytoplasm using a nuclear export signal (NES), was subcloned using standard procedures between the NheI and AscI restriction sites of the Cre recombinase-dependent AAV expression plasmid, Article Title: Social touch-like tactile stimulation activates a tachykinin 1-oxytocin pathway to promote social interactions. Article Snippet: Article Article Title: Regulation of hippocamposeptal input within the medial septum/diagonal band of Broca. Article Snippet: .. Mice were secured in a stereotax (Stoelting/Neurostar) using ear bars. Expressing:Article Title: [1902.06250] Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn Article Snippet: .. Production and intra-spinal injection of rAAV vectors The coding sequence for GCaMP3.NES, targeted to the cytoplasm using a nuclear export signal (NES), was subcloned using standard procedures between the NheI and AscI restriction sites of the Cre recombinase-dependent AAV expression plasmid, Plasmid Preparation:Article Title: [1902.06250] Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn Article Snippet: .. Production and intra-spinal injection of rAAV vectors The coding sequence for GCaMP3.NES, targeted to the cytoplasm using a nuclear export signal (NES), was subcloned using standard procedures between the NheI and AscI restriction sites of the Cre recombinase-dependent AAV expression plasmid, Article Title: Nerve Terminal Nicotinic Acetylcholine Receptors Initiate Quantal GABA Release from Perisomatic Interneurons by Activating Axonal T-Type (Cav3) Ca2+ Channels and Ca2+ Release from Stores Article Snippet: .. Plasmids pAAV-EF1a-double floxed-hChR2 (H134R)-mCherry-WPRE-HGHpA and Article Title: An interactive framework for whole-brain maps at cellular resolution Article Snippet: TVA, mCherry and RG were amplified from pCMMPTVA800 29 (Addgene plasmid 15778), pAAV--EF1adouble floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene plasmid 20297), and pHCMV-Rabies G 49 (Addgene plasmid 15785) by Phusion High-Fidelity DNA polymerase (Finnzymes, Finland), respectively. .. TVA, mCherry and RG were amplified from pCMMPTVA800 29 (Addgene plasmid 15778), Article Title: Social touch-like tactile stimulation activates a tachykinin 1-oxytocin pathway to promote social interactions. Article Snippet: Article Article Title: GluN2B-mediated regulation of silent synapses for receptor specification and addiction memory. Article Snippet: .. We expressed both photoactive (PA) and photoinsensitive (PI) Rac1 by cloning the PA-Rac1 (Addgene plasmid 22027) and PI-Rac1 (Addgene plasmid 22028) constructs into Article Title: Regulation of hippocamposeptal input within the medial septum/diagonal band of Broca. Article Snippet: .. Mice were secured in a stereotax (Stoelting/Neurostar) using ear bars. Amplification:Article Title: An interactive framework for whole-brain maps at cellular resolution Article Snippet: TVA, mCherry and RG were amplified from pCMMPTVA800 29 (Addgene plasmid 15778), pAAV--EF1adouble floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene plasmid 20297), and pHCMV-Rabies G 49 (Addgene plasmid 15785) by Phusion High-Fidelity DNA polymerase (Finnzymes, Finland), respectively. .. TVA, mCherry and RG were amplified from pCMMPTVA800 29 (Addgene plasmid 15778), Generated:Article Title: Social touch-like tactile stimulation activates a tachykinin 1-oxytocin pathway to promote social interactions. Article Snippet: Article Subcloning:Article Title: Social touch-like tactile stimulation activates a tachykinin 1-oxytocin pathway to promote social interactions. Article Snippet: Article Cloning:Article Title: GluN2B-mediated regulation of silent synapses for receptor specification and addiction memory. Article Snippet: .. We expressed both photoactive (PA) and photoinsensitive (PI) Rac1 by cloning the PA-Rac1 (Addgene plasmid 22027) and PI-Rac1 (Addgene plasmid 22028) constructs into Construct:Article Title: GluN2B-mediated regulation of silent synapses for receptor specification and addiction memory. Article Snippet: .. We expressed both photoactive (PA) and photoinsensitive (PI) Rac1 by cloning the PA-Rac1 (Addgene plasmid 22027) and PI-Rac1 (Addgene plasmid 22028) constructs into Mouse Assay:Article Title: Regulation of hippocamposeptal input within the medial septum/diagonal band of Broca. Article Snippet: .. Mice were secured in a stereotax (Stoelting/Neurostar) using ear bars. |