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paav ef1adouble  (Addgene inc)


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    Structured Review

    Addgene inc paav ef1adouble
    Paav Ef1adouble, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+ef1adouble/pAAV-EF1a-double+floxed-hChR2(H134R)-EYFP-WPRE-HGHpA+(Plasmid+%2320298)/pm41077338-58-27-34
    Average 96 stars, based on 545 article reviews
    paav ef1adouble - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Injection:

    Article Title: Leptin signaling in the dorsomedial hypothalamus couples breathing and metabolism in obesity.
    Article Snippet: DREADD (AAV8-hSyn-DIO-hM3D(Gq)-mCherry, Addgene, # 44361-AAV8, 43 1012 vg.mL 1) or Control (AAV8-hSyn-DIO-mCherry, Addgene, #50459-AAV8, 131013 vg.mL 1) virus was delivered bilaterally using pre-pulled glass micropipettes (Sutter, Novato, California) to the DMH using the following stereotactic coordinates from the animal’s bregma: 1.88 mm caudal, ±0.40 mm lateral, and 5.00 mm ventral. .. For optogenetic electrophysiology experiments, pAAV-EF1adouble floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV1) (R7 x 1012 vg.mL 1, Addgene, #20298-AAV1) was injected into the 16 Cell Reports 42, 113512, December 26, 2023 DMH. .. In a separate group LepRb-Cre DIO mice of animals, we administered a retrograde Cre-dependent caspase virus AAV2retro.EF1a.Flex.taCasp3.T2A.TEVp.WPRE.hGH (731012 vg.mL 1 Penn Vector Core – Addgene, #45580) in the dorsal raphe (DR) using the following stereotactic coordinates from the animal’s bregma: 4.6mm caudal and 3.0 ventral.

    Article Title: [1902.06250] Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn
    Article Snippet: .. Production and intra-spinal injection of rAAV vectors The coding sequence for GCaMP3.NES, targeted to the cytoplasm using a nuclear export signal (NES), was subcloned using standard procedures between the NheI and AscI restriction sites of the Cre recombinase-dependent AAV expression plasmid, pAAV-EF1adouble floxed-EYFP-WPRE-HGHpA, a gift from Karl Deisseroth (Addgene plasmid # 20296; http://n2t.net/addgene:20296; RRID:Addgene_20296). ..

    Sequencing:

    Article Title: [1902.06250] Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn
    Article Snippet: .. Production and intra-spinal injection of rAAV vectors The coding sequence for GCaMP3.NES, targeted to the cytoplasm using a nuclear export signal (NES), was subcloned using standard procedures between the NheI and AscI restriction sites of the Cre recombinase-dependent AAV expression plasmid, pAAV-EF1adouble floxed-EYFP-WPRE-HGHpA, a gift from Karl Deisseroth (Addgene plasmid # 20296; http://n2t.net/addgene:20296; RRID:Addgene_20296). ..

    Bioprocessing:

    Article Title: [1902.06250] Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn
    Article Snippet: .. Production and intra-spinal injection of rAAV vectors The coding sequence for GCaMP3.NES, targeted to the cytoplasm using a nuclear export signal (NES), was subcloned using standard procedures between the NheI and AscI restriction sites of the Cre recombinase-dependent AAV expression plasmid, pAAV-EF1adouble floxed-EYFP-WPRE-HGHpA, a gift from Karl Deisseroth (Addgene plasmid # 20296; http://n2t.net/addgene:20296; RRID:Addgene_20296). ..


    Article Title: Regulation of hippocamposeptal input within the medial septum/diagonal band of Broca.
    Article Snippet: .. Mice were secured in a stereotax (Stoelting/Neurostar) using ear bars. pAAV-EF1adouble floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene plasmid #20297 from Karl Deisseroth) was packaged with AAV stereotype 5 or 9 by the Viral Vector Core facility at the NIEHS and loaded into a Hamilton syringe with a 30-gauge needle (Hamilton Co.). ..

    Expressing:

    Article Title: [1902.06250] Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn
    Article Snippet: .. Production and intra-spinal injection of rAAV vectors The coding sequence for GCaMP3.NES, targeted to the cytoplasm using a nuclear export signal (NES), was subcloned using standard procedures between the NheI and AscI restriction sites of the Cre recombinase-dependent AAV expression plasmid, pAAV-EF1adouble floxed-EYFP-WPRE-HGHpA, a gift from Karl Deisseroth (Addgene plasmid # 20296; http://n2t.net/addgene:20296; RRID:Addgene_20296). ..

    Plasmid Preparation:

    Article Title: [1902.06250] Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn
    Article Snippet: .. Production and intra-spinal injection of rAAV vectors The coding sequence for GCaMP3.NES, targeted to the cytoplasm using a nuclear export signal (NES), was subcloned using standard procedures between the NheI and AscI restriction sites of the Cre recombinase-dependent AAV expression plasmid, pAAV-EF1adouble floxed-EYFP-WPRE-HGHpA, a gift from Karl Deisseroth (Addgene plasmid # 20296; http://n2t.net/addgene:20296; RRID:Addgene_20296). ..

    Article Title: Nerve Terminal Nicotinic Acetylcholine Receptors Initiate Quantal GABA Release from Perisomatic Interneurons by Activating Axonal T-Type (Cav3) Ca2+ Channels and Ca2+ Release from Stores
    Article Snippet: .. Plasmids pAAV-EF1a-double floxed-hChR2 (H134R)-mCherry-WPRE-HGHpA and pAAV-EF1adouble floxed-mCherry-WPRE-HGHpA were obtained from the plasmid-sharing site Addgene, through a material transfer agreement with Dr. K. Deisseroth (Stanford University, Stanford, CA). ..

    Article Title: An interactive framework for whole-brain maps at cellular resolution
    Article Snippet: TVA, mCherry and RG were amplified from pCMMPTVA800 29 (Addgene plasmid 15778), pAAV--EF1adouble floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene plasmid 20297), and pHCMV-Rabies G 49 (Addgene plasmid 15785) by Phusion High-Fidelity DNA polymerase (Finnzymes, Finland), respectively. .. TVA, mCherry and RG were amplified from pCMMPTVA800 29 (Addgene plasmid 15778), pAAV--EF1adouble floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene plasmid 20297), and pHCMV-Rabies G 49 (Addgene plasmid 15785) by Phusion High-Fidelity DNA polymerase (Finnzymes, Finland), respectively. ..


    Article Title: GluN2B-mediated regulation of silent synapses for receptor specification and addiction memory.
    Article Snippet: .. We expressed both photoactive (PA) and photoinsensitive (PI) Rac1 by cloning the PA-Rac1 (Addgene plasmid 22027) and PI-Rac1 (Addgene plasmid 22028) constructs into pAAV-EF1adouble floxed-hChR2(H134R)-eYFP-WPRE (Addgene plasmid 20298)21. ..

    Article Title: Regulation of hippocamposeptal input within the medial septum/diagonal band of Broca.
    Article Snippet: .. Mice were secured in a stereotax (Stoelting/Neurostar) using ear bars. pAAV-EF1adouble floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene plasmid #20297 from Karl Deisseroth) was packaged with AAV stereotype 5 or 9 by the Viral Vector Core facility at the NIEHS and loaded into a Hamilton syringe with a 30-gauge needle (Hamilton Co.). ..

    Amplification:

    Article Title: An interactive framework for whole-brain maps at cellular resolution
    Article Snippet: TVA, mCherry and RG were amplified from pCMMPTVA800 29 (Addgene plasmid 15778), pAAV--EF1adouble floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene plasmid 20297), and pHCMV-Rabies G 49 (Addgene plasmid 15785) by Phusion High-Fidelity DNA polymerase (Finnzymes, Finland), respectively. .. TVA, mCherry and RG were amplified from pCMMPTVA800 29 (Addgene plasmid 15778), pAAV--EF1adouble floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene plasmid 20297), and pHCMV-Rabies G 49 (Addgene plasmid 15785) by Phusion High-Fidelity DNA polymerase (Finnzymes, Finland), respectively. ..

    Generated:


    Subcloning:


    Cloning:

    Article Title: GluN2B-mediated regulation of silent synapses for receptor specification and addiction memory.
    Article Snippet: .. We expressed both photoactive (PA) and photoinsensitive (PI) Rac1 by cloning the PA-Rac1 (Addgene plasmid 22027) and PI-Rac1 (Addgene plasmid 22028) constructs into pAAV-EF1adouble floxed-hChR2(H134R)-eYFP-WPRE (Addgene plasmid 20298)21. ..

    Construct:

    Article Title: GluN2B-mediated regulation of silent synapses for receptor specification and addiction memory.
    Article Snippet: .. We expressed both photoactive (PA) and photoinsensitive (PI) Rac1 by cloning the PA-Rac1 (Addgene plasmid 22027) and PI-Rac1 (Addgene plasmid 22028) constructs into pAAV-EF1adouble floxed-hChR2(H134R)-eYFP-WPRE (Addgene plasmid 20298)21. ..

    Mouse Assay:

    Article Title: Regulation of hippocamposeptal input within the medial septum/diagonal band of Broca.
    Article Snippet: .. Mice were secured in a stereotax (Stoelting/Neurostar) using ear bars. pAAV-EF1adouble floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene plasmid #20297 from Karl Deisseroth) was packaged with AAV stereotype 5 or 9 by the Viral Vector Core facility at the NIEHS and loaded into a Hamilton syringe with a 30-gauge needle (Hamilton Co.). ..



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